After a goalless draw in the first leg in Lviv, the tie remains wide open, although the record German champions will fancy their chances of progressing to the last eight on home soil.
Bayern are among the favourites for the Champions League this season and will be keen to go at least as far as last year, when they reached the semi-finals. They need a win to go through while Shakhtar, who only reached the last eight once before, would go through with a score draw at the .
Philipp Lahm is now back in training having recovered from a fractured ankle but is not yet ready to make his comeback. He joins Javi Martinez and Thiago Alcantara on the sidelines, while Xabi Alonso (r.) is also unavailable due to suspension.
Bernard (l.) does not make the trip for Donetsk while Serhiy Kryvtsov and Ivan Ordets are doubtful. Taras Stepanenko is back after missing the first leg through suspension, though, giving coach Mircea Lucescu another option.
, Austria's sportsperson of the year the last two years running, has excelled in his defensive midfield role and can be sure to be involved heavily on Wednesday. And if there is a free-kick within 30 yards, step aside Arjen Robben and Franck Ribery, dead-ball specialist Alaba is Bayern's guarantee at present.
Luiz Adriano (l.) remains Shakhtar's dangerman, even if he did not get much of the ball in the first leg. His nine goals in the group stage – a joint competition record – were more than Thomas Müller, Arjen Robben and Robert Lewandowski have managed between them so far.
"We have to win tomorrow, this is very important. Shakhtar have forwards with quality that we have to be aware of. They're good on the counterattack, so we have to avoid this. Shakhtar are a good team, we know that. But, we're even better. Of course, our aim is to win the game and go through."
Mircea Lucescu (to UEFA.com): "In my opinion Bayern are the world's strongest football team at the moment. They have six world champions among their players, and leading figures like Ribéry or Robben. They are the strongest team in the world at the moment. And we are taking them very seriously, of course."
Bayern's goalless draw in Lviv punctuated a run of six straight wins which has extended their lead at the top of the Bundesliga to 11 points. They have conceded just two goals in those games, scoring 25. Shakhtar have resumed league duty since the first leg, winning two and drawing one of their first three games since the winter break.
Bayern have faced fellow Ukrainian outfit FC Dinamo Kyiv eight times, winning four out of four at home, but their clash with Shakhtar last month was their first meeting with the Pitmen, who have won only twice before in Germany. However, they did manage to defeat Bundesliga side SV Werder Bremen for their one and only European title - the UEFA Cup in 2009.
Bayern: Neuer - Rafinha, Boateng, Badstuber, Bernat - Schweinsteiger, Alaba - Robben, Müller, Ribéry - Lewandowski
Shakhtar: Pyatov - Srna, Kucher, Rakitskiy, Shevchuk - Stepanenko - Douglas Costa, Fernando, Alex Teixeira, Taison - Luiz Adriano