Armin Veh's side have already secured top spot in Group F having won four of their five matches, including a comfortable 3-0 victory over the Cypriot champions at the beginning of October.
With his team already in the hat for the knockout stages, Veh is set to make a number of changes to the side that suffered a 2-1 home defeat to 1899 Hoffenheim last weekend. One player likely to feature is Marco Russ, the 28-year-old defender having recovered from a thigh injury suffered against FC Schalke 04 on Matchday 13.
Much has been made of Frankfurt's contrasting fortunes in Europe and the Bundesliga this season, but with the Eagles sitting dangerously close to the relegation zone in the German top flight, Russ is under no illusions as to where their priorities lie. "The Leverkusen game [on Matchday 16] takes precedence, but that doesn't mean we're not taking Thursday's match seriously," he explained.
Reserve goalkeeper Felix Wiedwald may be given a chance to impress, while 18-year-old Marc-Oliver Kempf could partner Russ in central defence. "We may start with an unfamiliar line-up, but every player is determined to put in a good performance," said the former VfL Wolfsburg player. Croatian forward Srdjan Lakic may also be handed a rare starting berth in attack, while Japan international Takashi Inui could retain his place in midfield.
Veh offered plenty of encouragement to the more inexperienced members of his squad at the pre-match press conference on Wednesday: "If they play well, they might be selected in the league. We're determined to win the match, mainly due to the fact that 31,000 fans will be supporting us on Thursday, but it's nice that we can play without pressure."
Eintracht Frankfurt: Wiedwald – Schröck, Russ, Kempf, Djakpa – Lanig, Flum – Inui, Rosenthal, Barnetta – Lakic
APOEL: Urko – Mario Sergio, Borda, Marcelo Oliveira, Helder Cabral – Vinicius, Nuno Morais – Charalambidis, Solomou, Aloneftis – Manduca