The Wolves are looking to wrap up second spot behind FC Bayern München while Dortmund have a place in the UEFA Europa League still to seal, but both will have marked out this game as an opportunity for some reconnaissance ahead of their meeting in the DFB Cup final in Berlin on 30 May.
bundesliga.com takes a closer look at what is set to be anything but an end-of-season stroll at the Volkswagen Arena...
Wolfsburg are guaranteed UEFA Champions League football next term and are now jostling with Borussia Mönchengladbach for second place. Dortmund retain hopes of playing in Europe next season as well, which they can secure by finishing in the top six - or by beating Wolfsburg in a fortnight's time.
With one eye already on that meeting with Dortmund in Berlin, no risks will be taken with any players showing signs of fatigue. Marcel Schäfer, Luiz Gustavo and Ivan Perisic are carrying knocks but may still feature, however, while Xizhe Zhang (r.) could make his Bundesliga debut.
Marco Reus (l.) is fit again and set to get some minutes under his belt ahead of the final. Meanwhile, Sven Bender and Sebastian Kehl are the only two doubts for Jürgen Klopp, with Kehl set to miss what would be his penultimate Bundesliga appearance, having already announced his retirement in the summer.
With his 15th and 16th goals of the season last weekend, Bas Dost is now on a mission to end the season as the Bundesliga's top goalscorer. Bettering Alexander Meier's total of 19 is the aim in the final two games, and considering the Dutchman netted nine in four at the start of 2015, few would put it past him.
Henrikh Mkhitaryan may have scored fewer than some would have hoped this season, but he has been in fine fettle of late, assisting four in his last four games, three of which ended in victories, with one game drawn. "Micki's corners have been extremely good," said Klopp. "He's added a new feather to his cap."
Dieter Hecking, head coach: "This is going to be an extremely interesting game because Dortmund still need points to secure sixth and qualify for Europe. I expect them to come here with their strongest team, which should make for an attractive game."
Jürgen Klopp, head coach: "It's going to be a highly interesting game. The final is going to be something else - a special atmosphere. The only thing that will be the same will be the teams, perhaps. But if two teams have something to play for then it normally makes for a passionate game and I assume that’s what this will be."
Wolfsburg returned to winning ways against Paderborn after picking up only one point from their previous three games, although they did reach the DFB Cup final during that period. In the final, they will meet a Dortmund side whose recent revival has seen them climb from the foot of the table after the winter break to seventh, with the chance of going even higher.
This is the 36th game between these two sides and Wolfsburg have won only eight so far, losing 18 and drawing the other nine. One of those draws came just prior to the winter break, when Naldo's late header grabbed the Wolves a 2-2 draw in an entertaining encounter.
Wolfsburg: Benaglio - Träsch, Naldo, Klose, Rodriguez - Guilavogui, Luiz Gustavo - Perisic, De Bruyne, Caligiuri - Dost
Dortmund: Langerak - Durm, Subotic, Hummels, Schmelzer - Kehl, Gündogan - Mkhitaryan, Kagawa, Kampl - Aubameyang